CNS 14-3-3ζ: Changes with sex but not psychiatric diagnoses or psychotropic drug treatment

Abstract

mRNA for 14-3-3ζ, an abundant signalling protein in human CNS, is reported as decreased or unchanged in cortex from subjects with schizophrenia. Addressing this dichotomy, using Western blot analyses, we measured levels of 14-3-3ζ proteins in cortex and caudate nucleus from subjects with schizophrenia, bipolar disorder, age/sex matched controls and in analogous CNS regions from rats treated with psychotropic drugs. Anti-14-3-3ζ antibody bound to three proteins (molecular weights: 27, 54 and 70 kDa), in all CNS tissue. Levels of all proteins did not vary with diagnoses (27 kDa: F2,42.0 = 0.35, p = 0.71; 54 kDa: F2,42.1 = 0.62, p = 0.54; 70 kDa: F2,41.0 = 2.43, p = 0.10). By contrast, independ..